Inhibitory effect of ethyl acetate fraction from Cudrania tricuspidata on the expression of nitric oxide synthase gene in RAW 264.7 macrophages stimulated with interferon-gamma and lipopolysaccharide
Wg. Seo et al., Inhibitory effect of ethyl acetate fraction from Cudrania tricuspidata on the expression of nitric oxide synthase gene in RAW 264.7 macrophages stimulated with interferon-gamma and lipopolysaccharide, GEN PH-VASC, 35(1), 2000, pp. 21-28
It was found that the production of nitric oxide (NO) by RAW 264.7 macropha
ges stimulated with interferon-gamma (IFN-gamma) and lipopolysaccharide (LP
S) could be markedly inhibited by the ethyl-acetate-soluble fraction of 80%
aqueous methanolic extract of stem barks of Cudrania tricuspidata (EACT).
Inhibition of NO production was achieved by reducing inducible nitric oxide
synthase (iNOS) expression at protein and mRNA levels and by inactivating
nuclear factor-kappaB (NF-kappaB), but not by inhibiting iNOS activity. Thu
s, further phytochemical and pharmacological studies may lead to isolation
and structural identification of an inhibitor of iNOS from C tricuspidata,
which has been used as a traditional medicine for curing inflammation. (C)
2001 Elsevier Science Inc. All rights reserved.