Genome-wide ENU mutagenesis to reveal immune regulators

A complete list of molecular components for immune system function is now a vailable with the completion of the human and mouse genome sequences. Howev er, identification and functional annotation of genes involved in immunolog ical processes require a discovery methodology that can efficiently and bro adly analyze the complex interplay of these components in vivo. Our recent experience indicates that genome-wide chemical mutagenesis in the mouse is an extremely powerful methodology for the identification of genes required for complex immunological processes.