HIGH-SPEED ELECTROPHORETIC SEPARATION OF DNA FRAGMENTS USING A SHORT CAPILLARY

Authors
CHAN KC MUSCHIK GM ISSAQ HJ
Citation
Kc. Chan et al., HIGH-SPEED ELECTROPHORETIC SEPARATION OF DNA FRAGMENTS USING A SHORT CAPILLARY, Journal of chromatography B. Biomedical sciences and applications, 695(1), 1997, pp. 113-115
Citations number
11
Categorie Soggetti
Chemistry Analytical","Biochemical Research Methods
Journal title
Journal of chromatography B. Biomedical sciences and applications → ACNP
ISSN journal
13872273
Volume
695
Issue
1
Year of publication
1997
Pages
113 - 115
Database
ISI
SICI code
0378-4347(1997)695:1<113:HESODF>2.0.ZU;2-L
Abstract
Capillary electrophoresis using a replaceable gel buffer was applied t o the separation of DNA fragments. A short effective length capillary (1-2 cm) at low electric field allowed the separation of a 20-1000 bp ladder in 1 min. Although similar separation speed was achieved with a longer capillary at high held, the resolution of larger fragments was degraded. The short effective length capillaries were able to separat e the wildtype and mutant PCR products of the TGF-beta(1) gene in unde r 45 s.