CAPILLARY ELECTROPHORESIS OF PEPTIDES AND PROTEINS AT NEUTRAL PH IN CAPILLARIES COVALENTLY COATED WITH POLYETHYLENEIMINE

Authors
FIGEYS D AEBERSOLD R
Citation
D. Figeys et R. Aebersold, CAPILLARY ELECTROPHORESIS OF PEPTIDES AND PROTEINS AT NEUTRAL PH IN CAPILLARIES COVALENTLY COATED WITH POLYETHYLENEIMINE, Journal of chromatography B. Biomedical sciences and applications, 695(1), 1997, pp. 163-168
Citations number
14
Categorie Soggetti
Chemistry Analytical","Biochemical Research Methods
Journal title
Journal of chromatography B. Biomedical sciences and applications → ACNP
ISSN journal
13872273
Volume
695
Issue
1
Year of publication
1997
Pages
163 - 168
Database
ISI
SICI code
0378-4347(1997)695:1<163:CEOPAP>2.0.ZU;2-M
Abstract
Two procedures for the derivatization of the inner wall of fused-silic a capillaries for the analysis of peptides and proteins by capillary e lectrophoresis (CE) at neutral pH are presented. In the first procedur e, polyethyleneimine (PEI) is covalently attached to the capillary wal l. In the second procedure, PEI is additionally cross-linked. We prese nt analysis of standard peptides and proteins by CE using the coated c apillaries. These coatings will have application for the separation of protein complexes at neutral pH, prior to analysis by electrospray ma ss spectrometry.