MICELLAR ELECTROKINETIC CAPILLARY CHROMATOGRAPHY WITH LASER-INDUCED FLUOROMETRIC DETECTION OF AMINES IN BEER

Authors
PRESTON LM WEBER ML MURRAY GM
Citation
Lm. Preston et al., MICELLAR ELECTROKINETIC CAPILLARY CHROMATOGRAPHY WITH LASER-INDUCED FLUOROMETRIC DETECTION OF AMINES IN BEER, Journal of chromatography B. Biomedical sciences and applications, 695(1), 1997, pp. 175-180
Citations number
9
Categorie Soggetti
Chemistry Analytical","Biochemical Research Methods
Journal title
Journal of chromatography B. Biomedical sciences and applications → ACNP
ISSN journal
13872273
Volume
695
Issue
1
Year of publication
1997
Pages
175 - 180
Database
ISI
SICI code
0378-4347(1997)695:1<175:MECCWL>2.0.ZU;2-B
Abstract
Capillary electrophoresis with simultaneous ultraviolet absorbance and laser-induced fluorescence detection is applied to identify and quant ify selected amines in beer following derivatization with 7-chloro-4-n itrobenzo-2-oxa-1,3-diazole. Quantitation was performed using the meth od of standard addition in order to avoid pH-dependent variations in t he reactivity of the derivatizing agent with the added benefit of veri fying peak identity. An inexpensive and ''easy-to-use'' on-column fibe r optic fluorescence detection cell is described and implemented in th e analysis.