Cytotoxic effects of denture base materials on a permanent human oral epithelial cell line and on primary human oral fibroblasts in vitro

Purpose: The objective of this study was to determine the cytocompatibility of three different extracts of denture base resins and to compare the cyto toxic effect of these materials on a human oral epithelial KB cell line and primary human oral fibroblasts derived from buccal mucosa. Materials and M ethods: Set specimens from a heat-cured resin, a self-cured resin, and a li ght-cured resin were eluted with culture medium for 1, 3, and 5 days. Cytot oxicity was judged using tetrazolium bromide reduction assay. Results: The eluates from self-cured, heat-cured, and light-cured denture base resins we re cytotoxic to primary human buccal fibroblast cultures and KB cells, Self -cured resin was the most toxic denture base material among the chemicals t ested in all cultures. The cytotoxicity decreased in the order of self-cure d resin > heat-cured resin > light-cured resin for KB cells. The rank for b uccal fibroblast cells was self-cured resin > heat-cured resin > light-cure d resin. Conclusion: The influence of the cytotoxicity depended on the mate rials tested and the cell culture system used. The use of both permanent an d primary cells is recommended for a better screening of the cytotoxic effe cts of denture base resins.