Proliferation and release of IL-5 and IFN-gamma by peripheral blood mononuclear cells from cat-allergic asthmatics and rhinitics, non-cat-allergic asthmatics, and normal controls to peptides derived from Fel d 1 chain 1

Citation
Bm. Haselden et al., Proliferation and release of IL-5 and IFN-gamma by peripheral blood mononuclear cells from cat-allergic asthmatics and rhinitics, non-cat-allergic asthmatics, and normal controls to peptides derived from Fel d 1 chain 1, J ALLERG CL, 108(3), 2001, pp. 349-356
Citations number
37
Categorie Soggetti
Clinical Immunolgy & Infectious Disease",Immunology
Journal title
JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY
ISSN journal
00916749 → ACNP
Volume
108
Issue
3
Year of publication
2001
Pages
349 - 356
Database
ISI
SICI code
0091-6749(200109)108:3<349:PAROIA>2.0.ZU;2-N
Abstract
Background: In general, T cells from normal, nonatopic individuals respond to aeroallergens with synthesis and release of IFN-gamma. In contrast, rele ase of T(H)2-type cytokines by activated lymphocytes is a feature of allerg ic rhinitis and atopic asthma. Objective: The purpose of this study was to determine differences in T-cell recognition of epitopes within allergenic sequences, in terms of prolifera tion and cytokine production, in subjects with atopic asthma compared with subjects with allergic rhinitis and normal controls. Methods: Proliferative responses and IL-5/IFN-gamma release patterns from P BMCs from cat-allergic asthmatic, cat-allergic rhinitic, and non-cat-allerg ic asthmatic subjects and nonatopic normal controls were determined in prim ary cultures. Cells were challenged with 7 overlapping peptides spanning ch ain 1 of the major cat allergen, Fel d 1. Results: The 4 groups did not differ with respect to the ability to mount p roliferative responses to Fel d 1 peptides. In all groups, the IFN-gamma re sponses were predominantly to the amino terminus peptides. Cat-allergic and non-cat-allergic asthmatic subjects (and not cat-allergic rhinitic subject s and normal controls) made IL-5 responses to most of the Fel d 1 peptides, the result being a mixed (T(H)0) cytokine response at the N-terminus and a restricted (T(H)2) response at the C-terminus. Conclusion: Proliferative and IL-5/IFN-gamma responses of T cells from asth matic and atopic rhinitic subjects and normal controls to allergen peptides can be dissociated. Furthermore, differing cytokine responses to peptides derived from a single antigen suggest that certain domains of the molecule might preferentially induce IL-5 rather than IFN-gamma and as a result coul d be more important in disease pathogenesis.