Functional correlation between the nuclear localization of Fht1p and its flocculation and heat tolerance activities in budding yeast Saccharomyces cerevisiae

Fht1p is involved in the flocculation and heat tolerance machinery of buddi ng yeast Saccharomyces cerevisiae. Despite knowledge of its involvement in those phenotypes, a precise mechanism has yet to be discovered. To this end , we monitored the relationship between subcellular localization of Fht1p a nd its flocculation or heat tolerance function using newly developed expres sion vectors with a recombinant green fluorescent protein (GFP; S65T/S147P) of Aequorea victoria added at both the Nand C-terminus of Fht1p. The main fluorescent signal of the GFP tagged with either a wild-type Fht1p or mutan ts which preserve their flocculation function was detected in the nucleus, whereas signals of functionless mutants were dispersed to the cytoplasm. Co pyright (C) 2001 National Science Council, ROC and S. Karger AG, Basel.