Kf. Nielsen et U. Thrane, Fast methods for screening of trichothecenes in fungal cultures using gas chromatography-tandem mass spectrometry, J CHROMAT A, 929(1-2), 2001, pp. 75-87
The paper presents a fast method for trichothecene profiling and chemotaxon
omic studies in species of Fusarium, Stachybotrys, Trichoderma and Memnonie
lla. Micro scale extracted crude Fusarium extracts were derivatised using p
entafluoropropionic anhydride and analysed by gas chromatography with simul
taneous full scan and tandem mass spectrometric detection. It was possible
to monitor for up to four compounds simultaneous, making detection of acety
l T-2 toxin, T-2 toxin, HT-2 toxin, T-2 triol, T-2 tetraol, neosolaniol, is
o-neosolaniol, scirpentriol, 4,15-diacetoxyscirpenol, 15-acetoxyscirpenol,
4-acetoxyscirpentriol, nivalenol, fusarenon-X, deoxynivalenol, 15-acetyl-de
oxynivalenol and 3-acetyl-deoxynivalenol possible during a 23-min GC run. A
slightly modified method could detect trichothecenes produced by Stachybot
rys, Memnoniella and Trichoderma, by hydrolysing crude extracts prior to de
rivatisation with heptafluorobuturyl imidazole. All types of derivatised ex
tracts could be reanalysed using negative ion chemical ionisation (NICI) GC
-MS for molecular mass determination and verification purposes. A retention
time index could be used for correction in retention time drifts between s
equences and worked both in EI+ and NICI mode. (C) 2001 Elsevier Science B.
V. All rights reserved.