Application of analytical and preparative high-speed counter-current chromatography for separation of lycopene from crude extract of tomato paste

Authors
Wei, Y Zhang, TY Xu, GQ Ito, Y
Citation
Y. Wei et al., Application of analytical and preparative high-speed counter-current chromatography for separation of lycopene from crude extract of tomato paste, J CHROMAT A, 929(1-2), 2001, pp. 169-173
Citations number
20
Categorie Soggetti
Chemistry & Analysis","Spectroscopy /Instrumentation/Analytical Sciences
Journal title
JOURNAL OF CHROMATOGRAPHY A → ACNP
Volume
929
Issue
1-2
Year of publication
2001
Pages
169 - 173
Database
ISI
SICI code
Abstract
Lycopene was isolated from 100 mg of crude extract of tomato paste containi ng about 9% of lycopene. Analytical high-speed counter-current chromatograp hy (HSCCC) was first used for the systematic selection of the two-phase sol vent system. Then preparative HSCCC separation was performed with a nonaque ous solvent system composed of n-hexane-dichloromethane-acetonitrile at an optimum volume ratio of 10:3.5:6.5. This yielded 8.6 mg of lycopene at over 98.5% purity as determined by HPLC analysis. (C) 2001 Elsevier Science B.V . All rights reserved.