T. Murakami et al., Expression profiling of cancer-related genes in human keratinocytes following non-lethal ultraviolet B irradiation, J DERMA SCI, 27(2), 2001, pp. 121-129
Ultraviolet B irradiation initiates and promotes skin cancers, photo-aging,
and immune suppression. In order to elucidate the effect of these processe
s at the level of gene expression, we used cDNA microarray technology to ex
amine the effect of ultraviolet B irradiation on 588 cancer-related genes i
n human keratinocytes at 1, 6, and 24 h post-irradiation with a mildly cyto
toxic dose of ultraviolet B (170 mJ/cm(2)). The viability of the irradiated
keratinocytes was 75% at 24 h post-irradiation. Various cytokeratins and t
ranscription factors were up-regulated within 1 h post-irradiation. After 6
h, expression of a variety of genes related to growth regulation (e.g. p21
(WAFI), notch 4, and smoothened), apoptosis (e.g. caspase 10, hTRIP, and CR
AF1), DNA repair (ERCC1, XRCC1), cytokines (e.g. IL-6, IL-13, TGF-beta, and
endothelin 2), and cell adhesion (e.g. RhoE, and RhoGDI) were altered in h
uman keratinocytes. These data suggest the changes in a cascade of gene exp
ression in human keratinocytes occurring within 24 h after UVB exposure. Al
though the roles of these cellular genes after UVB-irradiation remain to be
elucidated, microarray analysis may provide a new view of gene expression
in epidermal keratinocytes following UVB exposure. (C) 2001 Elsevier Scienc
e Ireland Ltd. All rights reserved.