Apr. Sobrinho et al., Implantation of bacteria from human pulpal necrosis and translocation fromroot canals in gnotobiotic mice, J ENDODONT, 27(10), 2001, pp. 605-609
The aim of this study was to determine whether microorganisms recovered fro
m infected human root canals were able to survive and translocate to a loca
l lymph node when experimentally inoculated into the root canal system of g
erm-free mice. The microorganisms isolated from two patients with pulpal ne
crosis were inoculated in two groups of experimental animals; group I (Geme
lla morbillorum) and group II (Bifidobacterium adolescentis, Fusobacterium
nucleatum, and Clostridium butyricum). G. morbillorum showed the highest fr
equency of colonization and translocation to the draining lymph node. In gr
oup II only F. nucleatum and C. butyricum colonized and translocated when i
noculated in tri-association. When the bacteria from group II were inoculat
ed in monoinfection all three species colonized the root canal of germ-free
mice and translocated to the draining lymph node, but with different frequ
encies. We conclude that selective mechanisms occur in which some bacterial
species are fit to survive, multiply, and translocate in the germ-free mou
se model.