Inorganic nutrient utilisation by "adapted" Pseudomonas putida strain usedin the bioremediation of agricultural soil polluted with crude petroleum.

Garden soil samples polluted with crude petroleum were bioremediated by ino rganic nutrient monitoring with appropriate adjustment and inoculation with crude oil-adapted strain of Pseudomonas putida (PP) isolated from oil-impa cted soils. Soil samples without PP inoculation served as the control sampl es to compare the abilities of the native soil microflora with the adapted PP strain in biodegrading crude oil pollutant. In the experimental samples, oil concentration and all the inorganic nutrient sources tested decreased more rapidly with a proportional increase in the population densities of bo th PP and the native soil microflora than were observed in the control samp les. This trend was particularly strong for PO43- and NO3- which eventually became limiting both in all the experimental samples and in some control s amples, Inoculation of crude oil-impacted agricultural soils by oil -adapte d PP strain with nutrient monitoring and adjustment can be effective as bio remediation methods of agricultural land upon pollution with petroleum or p etroleum products.