Immunization with Haemophilus influenzae type b-CRM197 conjugate vaccine elicits a mixed Th1 and Th2CD4(+) T cell cytokine response that correlates with the isotype of antipolysaccharide antibody

Haemophilus influenzae type b (Hib) capsular polysaccharide (PS) induces pr otective antibodies but is T independent and poorly immunogenic in infants. Conjugate vaccines of Hib PS linked to proteins, such as CRM197, increase the PS antibody titer and elicit immunologic memory. To define the conjugat e-induced memory T cell response, 19 adults were immunized with Hib-CRM197, and antibody titers, carrier protein-specific CD4(+) T cell proliferation, and cytokine production were measured. Hib-CRM197 induced PS and CRM197 an tibodies, vigorous T cell recall responses, and production of cytokines, in cluding interleukin (IL)-2, IL-5,IL-10, and interferon-gamma. There was mar ked variability in PS antibody titer, despite consistent CRM197-specific re call responsiveness, which correlated with peak IgM and IgA PS antibody tit ers. Correlations were also found between IL-2 and IL-5 and IgA PS antibody levels. Hib-CRM197 induced a rapid increase in CRM197-specific memory T ce lls and mixed Th1/Th2 cytokines, which may regulate the isotype and quantit y of PS antibody.