We describe an innovative yet straightforward method to obtain high quality
thin sections of diatom exoskeletons for observation by scanning electron
microscopy (SEM). The use of this new technique allows for clear observatio
ns of some ultrastructural valve features, including the raphe, which are g
enerally difficult to observe and describe accurately using transmission el
ectron microscopy analysis of thin sections or SEM of randomly fractured di
atom valves. In addition, because this method involves the complete removal
of the organic content of the diatom cells, resulting in clean and mostly
undisturbed skeletal thin cross-sections, even the intact valvar structures
of weak girdle bands can be studied.