Synthesis and application of Fmoc-His(3-Bum)-OH

Authors
Mergler, M Dick, F Sax, B Schwindling, J Vorherr, T
Citation
M. Mergler et al., Synthesis and application of Fmoc-His(3-Bum)-OH, J PEPT SCI, 7(9), 2001, pp. 502-510
Citations number
15
Categorie Soggetti
Biochemistry & Biophysics
Journal title
JOURNAL OF PEPTIDE SCIENCE
ISSN journal
10752617 → ACNP
Volume
7
Issue
9
Year of publication
2001
Pages
502 - 510
Database
ISI
SICI code
1075-2617(200109)7:9<502:SAAOF>2.0.ZU;2-G
Abstract
This paper presents a reevaluation of the synthesis and properties of Fmoc- His(3-Bum)-OH regarding its application in SPPS with minimal racemization o f histidine residues during coupling and esterification reactions. By-produ ct formation during the deprotection of the test peptides could be signific antly reduced by scavenging the concomitantly formed HCHO, e.g. with methox yamine. Copyright (C) 2001 European Peptide Society and John Wiley & Sons. Ltd.