Intralesional granulocyte-monocyte colony-stimulating factor followed by subcutaneous interleukin-2 in metastatic melanoma: a pilot study in elderly patients

Aim and background Recent data in the literature indicate that antigen-pres enting cells (APC) are inactive in tumour tissue because of local immunosup pression. Tumour-infiltrating lymphocyte (TIL) signal activation transducin g mechanisms are also seriously impaired. Administration of granulocyte mac rophage-colony stimulating factor (GM-CSF) may lead to APC recovery and int erleukin (IL)-2 may restore local TIL activation. Moreover, IL-2 increases the systemic lymphocyte population, an event that seems to correlate with a better prognosis. Study design The present phase I-II study was carried out to examine whethe r intralesional injection of GM-CSF followed by subcutaneous IL-2 would ind uce a clinical response in advanced, pretreated elderly melanoma patients. Methods Sixteen patients over 60 years of age received intralesional. GM-CS F (150 ng per lesion on day 1), generally divided between the two largest c utaneous lesions, followed by perilesional subcutaneous IL-2 (3 000 000 IU) for 5 days (days 3 - 7 inclusive) every 3 weeks. Results Four clinical responses [two partial (PR) and two minimal (MR)] (25 %), which also involved lesions that had not been directly treated, and nin e cases of stable disease were observed. The response duration for PR and M R was 9, 4, 4 and 2.5 + months, respectively. Stable disease (56%) recorded in the nine patients was short-term (3 - 6 months). Three patients rapidly progressed after two, two and one therapy cycles, respectively. The patien t who reached the best PR had a fairly high absolute lymphocyte count (1600 -2400/mm(3)). The second one, who reached complete remission after subseque nt locoregional chemotherapy and hyperthermia, however, had a low absolute lymphocyte count that had doubled by the end of treatment. Blood lymphocyte values in the other patients were too varied to allow any correlation with clinical response. Therapy was well tolerated and only mild fever was obse rved, with the exception of one patient who had grade 3 fever, with muscle pain and arthralgia. Conclusions Considering the very low toxicity observed, this treatment migh t be indicated in elderly patients for whom systemic therapy is no longer a viable option. Improved scheduling and timing could result from further st udies.