Sequence-specific C-13 NMR assignments in a neurotoxin (candoxin) from Bungarus candidus

Authors
Parvathy, VR Chary, KVR Kini, RM Govil, G
Citation
Vr. Parvathy et al., Sequence-specific C-13 NMR assignments in a neurotoxin (candoxin) from Bungarus candidus, MAGN RES CH, 39(10), 2001, pp. 577-580
Citations number
14
Categorie Soggetti
Spectroscopy /Instrumentation/Analytical Sciences
Journal title
MAGNETIC RESONANCE IN CHEMISTRY
ISSN journal
07491581 → ACNP
Volume
39
Issue
10
Year of publication
2001
Pages
577 - 580
Database
ISI
SICI code
0749-1581(200110)39:10<577:SCNAIA>2.0.ZU;2-S
Abstract
We report C-13 NMR assignments of a weak neurotoxin called candoxin, using heteronuclear single quantum correlation (HSQC) experiments with their natu ral abundance in the protein. Almost complete sequence-specific C-13 NMR as signments were achieved. Chemical shift indices of C-13(alpha), C-13(beta) and H-1(alpha) were used to characterize the secondary structural segments in the protein. A consensus plot of chemical shift indices revealed five be ta -strands as the major structural elements in the toxin. Copyright (C) 20 01 John Wiley & Sons, Ltd.