Myeloperoxidase and adenosine-deaminase levels in the pleural fluid leakage induced by carrageenan in the mouse model of pleurisy

Background: Although myeloperoxidase (MPO) and adenosine-deaminase (ADA) le vels are markers of activated leukocytes, both enzymes have not been curren tly addressed in inflammation models. Aims: This study evaluates whether the concentrations of these enzymes are significantly correlated with the content of leukocytes in a pleurisy model . Methods: The pleurisy was induced by carrageenan (1%) in mice, and the para meters analyzed 4 and 48 h after. Results: After the induction of inflammation (4 h), MPO and ADA levels peak ed in parallel to neutrophils (p<0.01). Regarding the second phase of pleur isy (48 h), the highest concentrations of ADA were detected in parallel to the highest levels of mononuclears (p<0.01). At this time, MPO levels and n eutrophils; remained elevated, although at lower levels than those found at 4 h. A significant positive correlation was found among neutrophils and MP O, and mononuclears; and ADA (p<0.01). Conclusions: These findings support the evidence that both enzymes are mark ers of the inflammatory process, and provide new tools for a better underst anding of the immunoregulatory pathways that occur in inflammation.