Design and in vitro characterization of a single regulatory module for efficient control of gene expression in both plasmid DNA and a self-inactivating lentiviral vector

Background: Regulation of transgene expression in target cells represents a critical and challenging aspect of gene therapy. Recently, a two-plasmid t etracycline-inducible system was developed in which the tetracycline repres sor (tetR) alone, rather than the tetR-VP16 fusion derivative, was shown to function as a potent trans-modulator of a second plasmid that contains two tandem repeats of the tetracycline operator (tetO) inserted between the TA TA box and the transcription start site of the hCMV major immediate-early p romoter. A technological advance in this area would be the development of a single autoregulatory cassette that incorporates both of these components into nonviral and viral gene transfer vectors. For the latter, an inducible lentiviral vector that is capable of temporal and quantitative control of gene expression in either dividing or nondividing cells is highly desirable . Materials and Methods: A one-piece inducible (1Pi) autoregulatory cassette was constructed to provide IRES-mediated translation of the tetR as well as tight control over the tetO unit preventing transcription initiation of th e first cistron in the absence of the tetracycline. To increase efficiency of tetR-mediated repression, a nuclear localization signal was incorporated at the 3' end of the tetR gene. Regulation of gene expression at the trans criptional and protein level was analyzed in transient transfection experim ents using plasmid DNA. Construction of a self-inactivating lentiviral vect or containing this 1Pi cassette allowed the study of its long-term effectiv eness in primary human cells. Results: The 1Pi autoregulatory cassette when incorporated into plasmid DNA allows efficient control of the secretable hEGF as well as eGFP expression in a variety of cell types. Transient transfection studies demonstrated th at the time course of repression is different for the 1Pi and two-plasmid s ystem (2Pi). In the 2Pi system, greater repression is seen with the first 2 4-48 hr; however, by 72 hr, similar levels of repression with the 1Pi and 2 Pi systems are obtained. This regulation is reached three times faster when the tetR is modified with a nuclear localization signal to direct nascent proteins into the nuclear compartment. In addition, stable transduction of human umbilical vein endothelial cells (HUVEC) with a self-inactivating len tiviral vector incorporating this single regulator cassette provided tetrac ycline-inducible control of gene expression that is not diminished over tim e and is completely reversible upon removal of tetracycline. Conclusions: These results suggest a model in which the 1Pi autoregulatory system reaches a steady state over time, the minimal amount of tetR produce d by the basal activity of the CMV promoter and accumulated is adequate to replace the tetR that is lost over time. These studies also show that the i nducible self-inactivating lentiviral vector can temporally and reversibly regulate transgene expression in HUVECs. The use of this transcriptional co ntrol unit in both nonviral and viral vector delivery systems will constitu te an attractive technological advance for many gene therapy applications w here temporal and quantitative control of gene expression is desired. The s trengths and limitations of the 1Pi system are discussed.