ANALYSIS OF TRIGLYCERIDES BY CAPILLARY ELECTROCHROMATOGRAPHY

Capillary electrochromatography (CEC) on columns 25 and 40 cm long, 10 0 mu m i.d., and packed with 3 mu m Hypersil ODS was evaluated for the separation of triglycerides in different vegetable oils. The mobile p hase optimized for isocratic elution of triglycerides via micropacked liquid chromatography (micro-LC) and composed of acetonitrile/isopropa nol/n-hexane in the ratio 57/38/5 could be applied by adding 50 mM amm onium acetate. The influence of the voltage and the temperature on the resolution was studied. The possibilities of peak collection in CEC f or further elucidation of the triglyceride structure are illustrated w ith the identification of triolein in a salad oil by electrospray mass spectroscopy (ESMS). (C) 1997 John Wiley & Sons, Inc.