Modulating effect of Liv.100, an ayurvedic formulation on antituberculosisdrug-induced alterations in rat liver microsomes

The influence of Liv.100 on the hepatotoxicity of antituberculosis drugs [i soniazid (EH), rifampicin (RMP) pyrazinamide (PZA)] was studied in male alb ino rats. INH, RMP, and PZA were proved to be the most hepatotoxic. Rats we re treated with antituberculosis drugs daily for a period of 6 weeks by int ragastric administration. The combined use of antituberculosis drugs elevat ed the levels of cytochrome P-450 and cytochrome-b(5). A significant increa se was observed in the levels of NADPH-cytochrome P-450 reductase and NADH- cytochrome-bs reductases after antitubercular drug administration. During a ntitubercular drug treatment a significant decrease was also observed in th e activity of glucose-6-phosphatase. The extent of NADPH-induced and ascorb ic acid-induced lipid peroxides were marked in antitubercular drug treatmen t, when compared with normal control animals. Oral Liv.100 co-administratio n, for the same period, modulated the alterations in the xenobiotic metabol izing system and microsomal lipid peroxidation in experimental animals. The results are discussed with reference to drug metabolizing enzymes, lipid p eroxidation and the hepatoprotective nature of Liv.100. Copyright (C) 2001 John Wiley & Sons, Ltd.