Spatial distribution of leaf water-use efficiency and carbon isotope discrimination within an isolated tree crown

The spatial variations in the stable carbon isotope composition (delta C-13 ) of air and leaves (total matter and soluble sugars) were quantified withi n the crown of a well-watered, 20-year-old walnut tree growing in a low-den sity orchard. The observed leaf carbon isotope discrimination (Delta) was c ompared with that computed by a three-dimensional model simulating the intr acanopy distribution of irradiance, transpiration and photosynthesis (previ ously parameterized and tested for the same tree canopy) coupled to a bioph ysically based model of carbon isotope discrimination. The importance of di scrimination associated with CO2 gradients encountered from the substomatal sites to the carboxylation sites was evaluated. We also assessed by simula tion the effect of current irradiance on leaf gas exchange and the effect o f long-term acclimation of photosynthetic capacity and stomatal and interna l conductances to light regime on intracanopy gradients in Delta. The main conclusions of this study are: (i) leaf Delta can exhibit important variati ons (5 and 8 parts per thousand in total leaf material and soluble sugars, respectively) along light gradients within the foliage of an isolated tree; (ii) internal conductance must be taken into account to adequately predict leaf Delta and (iii) the spatial variations in Delta and water-use efficie ncy resulted from the short-term response of leaf gas exchange to variation s in local irradiance and, to a much lesser extent, from the long-term accl imation of leaf characteristics to the local light regime.