Cs. Fan et al., Phenylalanine biosynthesis in Brevibacterium lactofermentum using Escherichia coli genes pheA, aroG and tyrB, PROG NAT SC, 11(10), 2001, pp. 786-791
Genetic engineering technology to increase the production of L-phenylalanin
e was used in the study. Three genes encoding the key enzymes involved in t
he biosynthesis of L-phenylalanine were utilized, in which the gene aroG en
codes 3-deoxy-D-arabino-heptulosonate-7-phosphate synthetase ( DS); the gen
e phe.4 encodes bifunctional enzyme of chorisate mutase (CM) and prephenate
dehydratase (PD); and the gene tyrb encodes aminotransferase (AT). The thr
ee genes were amplified by polymerase chain reaction (PCR) from the genome
of the E. coli mutant strains resistant to fluro-DL-phenylalanine and inser
ted into the cloning vectors. Then, they were expressed in E. coli and Brev
ibacterium lactofermentum in a tandem arrangement. The expressed enzymes ha
d high activities in the host cells.