Phenylalanine biosynthesis in Brevibacterium lactofermentum using Escherichia coli genes pheA, aroG and tyrB

Genetic engineering technology to increase the production of L-phenylalanin e was used in the study. Three genes encoding the key enzymes involved in t he biosynthesis of L-phenylalanine were utilized, in which the gene aroG en codes 3-deoxy-D-arabino-heptulosonate-7-phosphate synthetase ( DS); the gen e phe.4 encodes bifunctional enzyme of chorisate mutase (CM) and prephenate dehydratase (PD); and the gene tyrb encodes aminotransferase (AT). The thr ee genes were amplified by polymerase chain reaction (PCR) from the genome of the E. coli mutant strains resistant to fluro-DL-phenylalanine and inser ted into the cloning vectors. Then, they were expressed in E. coli and Brev ibacterium lactofermentum in a tandem arrangement. The expressed enzymes ha d high activities in the host cells.