Immunolocalization of alkaloids and X-ray microanalysis of elements in lupin seeds

Immunolocalization. of alkaloids in lupin seeds (Lupinus spp.) has been per formed by cryofixation and conventional methods. Alkaloids were localized i n the protein bodies of the cotyledon cells. Some immunogold particles in t he walls of these cells were also observed. There were no differences in th e sites of localization between the two mentioned methods. X-ray microanaly sis of elements showed the presence of P, Mg, S, and K in the protein bodie s of cotyledon cells in lupin seeds. The role of K+ in alkaloids transport is discussed.