A tetraploid F-2 progeny segregating for resistance to black spot, grower h
abit, and absence of prickles on the stem and petioles was used to construc
t genetic linkage maps of rose. The F-1 of the progeny, 90-69, was created
by crossing a black spot-resistant amphidiploid, 86-7, with a susceptible t
etraploid, 82-1134. The F-1 was open-pollinated to obtain 115 seedlings. AF
LP and SSR markers were used to eliminate seedlings produced through cross-
fertilization. The remaining progeny set of 52 F-2 plants was used to study
the inheritance of 675 AFLPs, one isozyme, three morphological and six SSR
markers. AFLP markers were developed with three combinations of restrictio
n enzymes, EcoRI/MseI, KpnI/MseI and PstI/MseI. Most of the markers appear
to be in simplex or single-dose and segregated 3:1 in the progeny. One link
age map was constructed for each parent using only the single-dose markers.
The map of 86-7 consists of 171 markers assigned to 15 linkage groups and
covering more than 902 cM of the genome. The map of 82-1134 consists of 167
markers assigned to 14 linkage groups and covering more than 682 cM of the
genome. In the AFLP analysis, EcoRI/MseI generated nearly twice as many ma
rkers per run than PstI/MseI. Markers developed with three restriction enzy
me combinations showed a mixed distribution throughout the maps. A gene con
trolling the prickles on the petiole was located at the end of linkage grou
p 7 on the map of 86-7. A gene for malate dehydrogenase locus 2 was located
in the middle of linkage group 4 on the map of 86-7. These first-generatio
n maps provide initial tools for marker-assisted selection and gene introgr
ession for the improvement of modern tetraploid roses.