AN EFFICIENT COST-EFFECTIVE PROTOCOL FOR AUTOMATED FLUORESCENT-DNA SEQUENCING

A simple and cost-effective method is described to sequence double-str anded DNA (< 100 ng) using a very small amount of fluorescent sequenci ng mix (one-tenth of manufacturer-recommended amount). The modificatio n has significant advantages over conventional protocols and can be us ed to sequence, using a single sequencing kit, at least ten times the number of samples. This modified method also removes the post-reaction cleaning protocol and can be used for direct loading of samples on ge ls after completion of sequencing reactions. The accuracy of DNA seque ncing data from analysis of 700 to 750 nucleotides is greater than 98. 5%.