IL-1 beta and IL-8 in human fetal membranes: Changes with gestational age,labor, and culture conditions

PROBLEM: Interleukin (IL)-1 beta and IL-8 are associated with labor. This s tudy aimed to characterize their concentrations in fetal membranes and any changes in these with advancing gestation and to define as to whether there are interactions between the membranes in their expression. METHOD OF STUDY: mRNA and protein content of amnion and choriodecidua at in creasing gestations and before and after labor at term were quantified. Mem branes were also collected before and after labor, separated, and cultured. Protein production was measured by ELISA. RESULTS: IL-1 beta and IL-8 concentration increased in third trimester amni on and choriodecidua. Further increased expression of mRNA of both cytokine s was found after labor in both membranes except IL-8 production by amnion. Choriodecidua produced more of each cytokine than amnion, however, no inte raction between the membranes was demonstrated by culture. CONCLUSIONS: Increasing expression of IL-1 beta and IL-8 in amnion and chor iodecidua in the third trimester and after labor supports a role for these cytokines in the establishment of labor.