Monitoring biogenic volatile compounds emitted by Eucalyptus citriodora using SPME

A procedure to monitor BVOC emitted by living plants using SPME technique i s presented. For this purpose, a glass sampling chamber was designed. This device was employed for the characterization of biogenic volatile compounds emitted by leaves of Eucalyptus citriodora. After extraction with SPME fib ers coated wi th PDMS/ DVB, it was possible to identify or detect 33 compou nds emitted by this plant. A semiquantitative approach was applied to monit or the behavior of the emitted BVOC during 9 days. Circadian profiles of th e variation in the concentration of isoprene were plotted. Using diffusion- based SPME quantitation, a recently introduced analytical approach, with ex traction times as short as 15 s, it was possible to quantify subparts-per-b illion amounts of isoprene emitted by this plant.