ASSIGNMENT OF THE HUMAN GENE FOR KBF2 RBP-JK TO CHROMOSOME 9P12-13 AND 9Q13 BY FLUORESCENCE IN-SITU HYBRIDIZATION/

The transcription factor KBF2 has been characterized as a factor that binds to the NFkB site of mouse major histocompatibility complex (MHC) class I genes and its amino acid sequence has been shown to be identi cal to those of members of the recombination signal-sequence binding p rotein (RBP-Jk) family. Previous studies by Amakawa ct al, (Genomics 1 7, 306-315, 1993) demonstrated that the functional gene is localized a t human chromosome 3q25. However, in the present study we showed by in situ hybridization with the functional KBF2/RBPJk cosmid clone that t he gene is localized at 3p12-13 and 9q13, namely, at the same loci as pseudogenes that were reported previously (Zhang et al., Jpn J Human G enet 39, 391-401, 1994).