Nitric oxide: An unconventional messenger in the nervous system of an orthopteroid insect

Nitric oxide (NO) is a membrane-permeant messenger molecule generated from the amino acid L-arginine. NO can activate soluble guanylyl cyclase leading to the formation of cyclic GMP (cGMP) in target cells. In the nervous syst em, NO/cGMP signalling is thought to play essential roles in synaptic plast icity during development and also in the mature animal. This paper examines biochemical, cell biological, and physiological investigations of NO/cGMP signalling in the nervous system of the locust, a commonly used neurobiolog ical preparation. Biochemical investigations suggest that an identical enzy me is responsible for both NO synthase (NOS) and NADPH-diaphorase activity after tissue fixation. Immunocytochemical staining of an olfactory center i n the locust brain shows that NOS-immunoreactivity colocalizes with NADPH-d iaphorase at the cellular level. The cytochemical staining of NO donor and target cells in adult animals suggests functions in olfaction, vision, and sensorimotor integration. During development, NO is implicated in axonal ou tgrowth and synaptogenesis. The cellular distribution of NO-responsive cell s in neural circuits reflects potential functions of NO as a retrograde syn aptic messenger, as an intracellular messenger, and as a lateral diffusible messenger independent of conventional synaptic connectivity. (C) 2001 Wile y-Liss, Inc.