A clear relationship was observed between the adhesion of Thiobacillus
ferrooxidans and Thiobacillus thiooxidans to different substrates and
the nutrient composition of the culture medium used for growth. Howev
er, a linear relationship between the percentage of attachment and the
efficiency of bioleaching by these microorganisms was not found. The
direct attack mechanism on insoluble sulphides by Thiobacillus ferroox
idans was confirmed in the absence of iron. (C) 1997 Elsevier Science
Ltd.