F. El-shintinawy et Mn. El-shourbagy, Alleviation of changes in protein metabolism in NaCl-stressed wheat seedlings by thiamine, BIOL PLANT, 44(4), 2001, pp. 541-545
NaCl-stress induced a pronounced suppression in growth of wheat seedlings.
The most abundant amino acids (cysteine, arginine, methionine) constituting
about 55 % of total free amino acid content in control wheat were reduced
in 100 mM NaCl-treated plants. However, valine, isoleucine, aspartic acid a
nd proline accumulated in response to NaCl stress and NaCl-treated wheat se
edlings showed 1.6 fold increase in total free amino acids compared to the
control. Addition of 2 muM thiamine alleviated the effects of NaCl on the a
mino acid composition and the amount of total free amino acids decreased to
that in the control. Content of 26 kDa protein increased in NaCl-treated p
lants, stimulation was more pronounced in roots than in shoots. In contrast
, the contents of 13 and 20 kDa proteins decreased. After addition of thiam
ine, the 24 kDa protein, which disappeared with NaCl treatment, has been in
itiated again. Moreover, thiamine treatment stimulated the accumulation of
the 20 kDa protein.