Magnetic resonance image attributes of the ovarian follicle wall during development and regression

We analyzed image characteristics in T-1-, T-2-, and diffusion-weighted in vitro magnetic resonance (MR) images acquired at predefined stages of the o varian cycle in 36 heifers to test the hypothesis that MR image attributes of the follicle wall reflect the physiologic status of ovarian follicles (v iable, atretic, dominant, subordinate). Numerical pixel values (NPV), stand ard deviation of pixel values (heterogeneity), and area under the curve wer e used to assess images of follicle walls. Pixel values of the wall were us ed to calculate a regression line from which intercept, slope, and coeffici ent of determination were calculated. In T-1 images, NPV of dominant follic les were less likely to fit a regression line at the preovulatory phase tha n at any other phase (P < 0.1). Preovulatory dominant follicles had lower a rea under the curve in diffusion-weighted images than early and late static dominant follicles of the anovulatory wave (P < 0.02). Subordinate follicl es in the presence of a preovulatory dominant follicle had lower mean NPV i n T-1- and T-2-weighted images and lower intercepts in T-1-weighted images than subordinate follicles of the anovulatory wave (P < 0.02). Early atresi a of dominant follicles was identified at the late static phase by greater area, mean NPV, and slope in T-2-weighted images (P < 0.02). Preovulatory d ominant follicles had poor fit of NPV to a regression line in T-1-weighted images and lower area under the curve in diffusion images. Atretic follicle s had brighter walls with more acute transitions from follicular fluid to s troma in T-2-weighted images and more heterogeneous walls in diffusion imag es. The MR image attributes of the follicle wall reflected the physiologic status of dominant and largest subordinate follicles.