Selection of genomic target RNAs by iterative screening

A growing number of proteins are known to exert their regulatory or biologi cal functions via RNA binding. In some cases genetic interactions allow us to infer candidate targets for RNA directed regulation, but in many other c ases identification of potential regulatory targets is problematic. We have developed an in vitro biochemical screen, SETIS (SElection of < < genomic > > Target RNAs by Iterative Screening) that allows screening of a major po rtion of the genome for identification of potential targets for RNA binding proteins. (C) 2001 Elsevier Science Ltd. All rights reserved.