Expression and purification of Listeria innocua Sv6b p60 invasion associated protein

The p60 protein of Listeria is a major extra-cellular protein which is used as indicator for the detection of these bacteria from contaminated food sa mples. To produce p60 in Escherichia coli, the invasion associated protein (iap) gene of L. innocua Sv6b encoding p60 was cloned and over-expressed wi th expression vector pMAL-C2. Recombinant pMBP-iap/innocua was induced with IPTG in E. coli. The expressed recombinant p60 protein that was fused with a maltose-binding protein (MBP) was purified by amylose resin-based affini ty chromatography. The purified recombinant p60 protein was also detected a s denatured and neutralized form by using a specific p60 monoclonal antibod y against L. monocytogenes and it may be useful for the production of L. in nocua-specific antibody.