D. Centonze et al., Adenosine-mediated inhibition of striatal GABAergic synaptic transmission during in vitro ischaemia, BRAIN, 124, 2001, pp. 1855-1865
Several reports have shown that energy deprivation, as a result of hypoxia,
hypoglycaemia or ischaemia, depresses excitatory synaptic transmission in
virtually all brain areas. How this pathological condition affects inhibito
ry synaptic transmission is still unclear. In the present in vitro study, w
e coupled whole-cell patch clamp recordings from striatal neurones with foc
al stimulation of GABAergic nerve terminals in order to characterize the el
ectrophysiological effects of combined oxygen and glucose deprivation (in v
itro ischaemia) on inhibitory postsynaptic currents (IPSCs) in this brain a
rea. We found that brief periods (2-5 min) of in vitro ischaemia invariably
caused a marked depression of IPSC amplitude. This inhibitory effect was f
ully reversible on removal of the ischaemic challenge. It was coupled with
an increased paired-pulse facilitation, suggesting the involvement of presy
naptic mechanisms. Accordingly, the ischaemic inhibition of striatal GABAer
gic IPSCs was not caused by a shift in the reversal potential of GABA(A)-re
ceptor mediated synaptic currents, and was independ- ent of postsynaptic AT
P concentrations. Endogenous adenosine, acting on A1 receptors, appeared re
sponsible for this presynaptic action as the ischaemic depression of IPSCs
was prevented by CPT [8-(4-chlorophenylthio) adenosine] and DPCPX, two aden
osine A1 receptor antagonists, and mimicked by the application of adenosine
in the bathing solution. Conversely, ATP-sensitive potassium channels were
not involved in the inhibition of IPSCs by ischaemia, as demonstrated by t
he fact that tolbutamide and glipizide, two blockers of these channels, wer
e ineffective in preventing this electrophysiological effect. The early dep
ression of GABA-mediated synaptic transmission might play a role in the dev
elopment of irreversible neuronal injury in the course of brain ischaemia.