We quantitatively evaluate the changes of the proliferative cell population
s in the adult tench retinas maintained at 6 degreesC and 20 degreesC by bo
th PCNA antigen detection and flow cytometry-based DNA measurements. Both t
he overall percentage of S-phase cells, in the whole retinas and the number
of PCNA-positive cells in each of the retinal layers were significantly lo
wer in the tench kept at 6 degreesC, indicating that temperature affects th
e retinal germinal cell proliferation. (C) 2001 Elsevier Science B.V. All r
ights reserved.