Supercritical fluid extraction of ethoxyquin from a beef matrix

The extraction and determination of ethoxyquin is becoming increasingly imp ortant as products, which are labeled as "natural" are becoming more common . This manuscript investigates the use of supercritical fluids in the extra ction of ethoxyquin from lean beef and beef fat. Three fluids were examined : carbon dioxide, trifluoromethane, and 1,1,1,2-tetrafluoroethane. Carbon d ioxide appeared to react with ethoxyquin during the extraction. Methanol mo dified hydrofluorocarbons provided more complete extractions over pure hydr ofluorocarbon fluids. Methanol modified 1,1,1,2-tetrafluoroethane was used in the extraction of ethoxyquin from lean beef and beef fat, and provided a quantitative extraction at the 0.5 ppm level.