Sr. Sturzenbaum et P. Kille, Control genes in quantitative molecular biological techniques: the variability of invariance, COMP BIOC B, 130(3), 2001, pp. 281-289
Citations number
48
Categorie Soggetti
Biochemistry & Biophysics
Journal title
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY
The measurement of transcript levels constitutes the foundation of today's
molecular genetics. Independent of the techniques used, quantifications are
generally normalised using invariant control genes to account for sample h
andling, loading and experimental variation. All of the widely used control
genes are evaluated, dissecting different methodological approaches and is
sues regarding the experimental context (e.g. development and tissue type).
Furthermore, the major sources of error are highlighted when applying thes
e techniques. Finally, different approaches undertaken to assess the invari
ance of control genes are critically analysed to generate a procedure that
will help to discern the best control for novel experiments. (C) 2001 Elsev
ier Science Inc. All rights reserved.