T-cell subtypes in bronchoalveolar lavage fluid and in peripheral blood from patients with primary lung cancer

The changes in local immunology play an important role in lung cancer devel opment. Ve used bronchoalveolar lavage fluid (BALF) and peripheral blood (P B) for the analysis of cell profiles in patients with primary lung cancer. Twenty-one patients with confirmed primary lung cancer and 13 healthy volun teers were investigated. All persons were smokers. The analysis of T-cell s ubsets was performed with a flow cytometry method and with the following an tibodies: anti CD3, CD4, CD8, CD16, CD25, CD-45, CD56, and HLA-DR. We found differences in the proportion of lymphocytes between BALF and PB, and a hi gher proportion of T cells and a lower proportion of B and natural-killer ( NK) cells in BALF. There was a significant difference in the proportion of T-cytotoxic/suppressor lymphocytes, which was elevated in the BALF of patie nts and decreased in patients' PB. The T-helper:cytotoxic/suppressor (Th:Tc /s) ratio was significantly lower in the BALF of patients. These changes we re visible in patients with a small cell type. The percentage of T cells wi th the a chain of receptor to IL-2 (IL-R) was lower in the BALF of patients than in the control group. Our observations reflect local changes in lung environment in patients affected with lung cancer.(C) 2001 Wiley-Liss, Inc.