Enantioseparation of the anticoagulant drug phenprocoumon in capillary electrophoresis with UV and laser-induced fluorescence detection and application of the method to urine samples

The enantioseparation of phenprocoumon (PhC) in capillary electrophoresis ( CE) has been studied using various cyclodextrins (CDs) such as native alpha , beta and gamma -CD and several neutral and randomly, as well as selective ly substituted charged CD derivatives. Reversal of the enantiomer migration order was observed when using heptakis(2,3,6-tri-O-methyl (TM)-beta -CD as a chiral selector compared to all other CDs used. The detection of PhC was performed using either UV or laser-induced fluorescence (LIF) detection. T he limit of detection (LOD) observed with LIF detection was ca. 20 times lo wer compared to UV. The method has been applied to the analysis of urine sa mples of the patient under treatment with PhC in combination with other dru gs such as ramipril, hydrochlorothiazide, and nifedipine.