Effect of organic solvent, electrolyte salt and a loading of cellulose tris (3,5-dichlorophenyl-carbamate) on silica gel on enantioseparation characteristics in capillary electrochromatography

The effect of the amount of the chiral selector, cellulose tris(3,5-dichlor ophenylcarbamate) (CDCPC) on the separation characteristics of enantiomers of some charged and neutral analytes was studied in capillary electrochroma tography (CEC). For better understanding of the effect of the loading of th e chiral selector on the particles and pore size of the packing material, l aser-beam particle size analyzer and scanning electron microscopy (SEM) wer e used. As shown in this study, CDCPC can be used for CEC enantioseparation s of a wide range of chiral charged and neutral analytes with high efficien cy. The loading of the polysaccharide derivative on the surface of silica m aterials even in high amounts does not markedly affect the particle size an d porous structure of the packing material. The separation characteristics are strongly affected by the loading of CDCPC onto silica gel in both CEC a nd capillary liquid chromatography (CLC).