Identification for H-villosa chromatin in wheat lines using genomic in situ hybridization, C-banding and gliadin electrophoresis techniques

Detection of H. villosa chromosomes in telosomic addition and translocation lines of common wheat was undertaken using genomic in situ hybridization ( GISH), C-banding techniques and polyacrylamide gels electrophoresis. The re sult of GISH on mitotic metaphase cells of the addition line '95039' indica ted that the added telochromosomes originated from H. villosa, and it was p robably 6VS or 7Vs of H. villosa according to the C-banding pattern. Furthe rmore, the analysis of gliadin profiles demonstrated that the telochromosom e was 6VS. A pair of 1RS/1BL translocation chromosome was also found in '95 039'. In addition, mitotic GISH analysis showed that the 6VS/6AL translocat ion chromosome remained unchanged after being transferred into new wheat ba ckground.