Inhibition by docosahexaenoic acid of receptor-mediated Ca2+ influx in ratvascular smooth muscle cells stimulated with 5-hydroxytryptamine

Citation
M. Hirafuji et al., Inhibition by docosahexaenoic acid of receptor-mediated Ca2+ influx in ratvascular smooth muscle cells stimulated with 5-hydroxytryptamine, EUR J PHARM, 427(3), 2001, pp. 195-201
Citations number
37
Categorie Soggetti
Pharmacology & Toxicology
Journal title
EUROPEAN JOURNAL OF PHARMACOLOGY
ISSN journal
00142999 → ACNP
Volume
427
Issue
3
Year of publication
2001
Pages
195 - 201
Database
ISI
SICI code
0014-2999(20010921)427:3<195:IBDAOR>2.0.ZU;2-G
Abstract
The effect of docosahexaenoic acid treatment on intracellular Ca2+ dynamics in rat vascular smooth muscle cells stimulated with 5-hydroxytryptamine (5 -HT) has been investigated in order to elucidate one of the mechanisms for its beneficial effect on cardiovascular disorders. The treatment of cells w ith 30 muM docosahexaenoic acid for 2 days inhibited an increase in intrace llular Ca2+ concentration induced by 5-HT (10 muM) and a depolarizing conce ntration of KCI (80 mM). Docosahexaenoic acid treatment significantly inhib ited divalent cation influx stimulated by 5-HT and KCI, as measured by Mn2 quenching method, whereas had no effect on 5-HT-induced Ca2+ release from the internal stores. Docosahexaenoic acid treatment also significantly inhi bited 5-HT receptor-mediated Ca2+ influx through Ni2+-insensitive channels that were distinct from store-operated channels. These results suggest that the specific inhibition of intracellular Ca2+ dynamics in vascular smooth muscle cells may contribute to the beneficial properties of docosahexaenoic acid on cardiovascular disorders. (C) 2001 Elsevier Science B.V. All right s reserved.