VE-cadherin-derived cell-penetrating peptide, pVEC, with carrier functions

Cell-penetrating peptides, CPPs, have been shown to translocate into living cells by a receptor-independent mechanism and to carry macromolecules over the plasma membrane. This article reports studies of the internalization o f pVEC, an 18-amino acid-long peptide derived from the murine sequence of t he cell adhesion molecule vascular endothelial cadherin, amino acids 615-63 2. Fluorophore-labeled pVEC entered four different cell lines tested: human aortic endothelial cells, brain capillary endothelial cells, Bowes melanom a cells, and murine brain endothelial cells. In order to evaluate the trans location efficiency of pVEC, we performed a side-by-side comparison with pe netratin, a well-characterized CPP. The cellular uptake of pVEC was highest for murine brain endothelial cells. All cell lines tested contained equal or slightly higher concentrations of pVEC than penetratin. pVEC mainly accu mulated in nuclear structures but was also found throughout the cells. Furt hermore, pVEC functioned as a transporter of both a hexameric peptide nucle ic acid molecule of 1.7 kDa and a 67-kDa protein, streptavidin-FITC, and ce llular uptake of fluorophore-labeled pVEC took place at 4 degreesC, suggest ing a nonendocytotic mechanism of translocation. In conclusion, our results indicate that pVEC is efficiently and rapidly taken up into cells and func tions as a potent carrier peptide. (C) 2001 Academic Press.