Localisation of mRNAs for diamine oxidase and histamine receptors H1 and H2, at the feto-maternal interface of human pregnancy

Objective and design: To localise mRNAs for the histamine receptors H1, H2 and H3, and for diamine oxidase, in the placenta and decidua of the human f eto-maternal interface. Materials and methods: Complementary DNA for each mRNA of interest was ampl ified by polymerase chain reaction. Sub-cloned sequences were used to prepa re probes for in situ hybridisation, and these were employed to localise th e expression of mRNAs for histamine receptors H1 and H2, and for diamine ox idase. Results: mRNA for histamine receptors H1 and H2, and for diamine oxidase co uld be detected at the feto-maternal interface of human pregnancy, and loca lised to both decidual and placental cells. Conclusion: The co-expression of these receptors and DAO is consistent with a role for histamine at the feto-maternal interface of human pregnancy.