Dense core secretory vesicles revealed as a dynamic Ca2+ store in neuroendocrine cells with a vesicle-associated membrane protein aequorin chimaera

The role of dense core secretory vesicles in the control of cytosolic-free Ca2+ concentrations ([Ca2+](c)) in neuronal and neuroendocrine cells is eni gmatic. By constructing a vesicle-associated membrane protein 2-synaptobrev in.aequorin chimera, we show that in clonal pancreatic islet beta -cells: ( a) increases in [Ca2+](c) cause a prompt increase in intravesicular-free Ca 2+ concentration ([Ca2+](SV)), which is mediated by a P-type Ca2+-ATPase di stinct from the sarco(endo) plasmic reticulum Ca2+-ATPase, but which may be related to the PMR1/ATP2C1 family of Ca2+ pumps; (b) steady state Ca2+ con centrations are 3-5-fold lower in secretory vesicles than in the endoplasmi c reticulum (ER) or Golgi apparatus, suggesting the existence of tightly bo und and more rapidly exchanging pools of Ca2+; (C) inositol (1,4,5) trispho sphate has no impact on [Ca2+](SV) in intact or permeabilized cells; and (d ) ryanodine receptor (RyR) activation with caffeine or 4-chloro-3-ethylphen ol in intact cells, or cyclic ADPribose in permeabilized cells, causes a dr amatic fall in [Ca2+](SV). Thus, secretory vesicles represent a dynamic Ca2 + store in neuroendocrine cells, whose characteristics are in part distinct from the ER/Golgi apparatus. The presence of RyRs on secretory vesicles su ggests that local Ca2+-induced Ca2+ release from vesicles docked at the pla sma membrane could participate in triggering exocytosis.