L. Monteiro et al., Magnetic immuno-PCR assay with inhibitor removal for direct detection of Helicobacter pylori in human feces, J CLIN MICR, 39(10), 2001, pp. 3778-3780
A PCR protocol was developed to detect Helicobacter pylori in human stool s
pecimens. This protocol was based on the association of a magnetic immuno-P
CR assay with a technique to remove inhibitors (agarose-embedded DNA prepar
ation). Of the 47 H. pylori-positive and 57 H. pylori-negative patients inc
luded in this study, 38 were positive and 66 were negative by this new prot
ocol. The sensitivity, specificity, and predictive values for a positive or
a negative result were 80.9% (95% confidence interval [CI], 66.3 to 90.4),
100% (95% CI, 92.1 to 100), 100% (95% CI, 88.6 to 100), and 86.4% (95% CI,
75.2 to 93.2), respectively.