Removal of PCR inhibitors by silica membranes: Evaluating the amplicor Mycobacterium tuberculosis kit

Authors
Boddinghaus, B Wichelhaus, TA Brade, V Bittner, T
Citation
B. Boddinghaus et al., Removal of PCR inhibitors by silica membranes: Evaluating the amplicor Mycobacterium tuberculosis kit, J CLIN MICR, 39(10), 2001, pp. 3750-3752
Citations number
34
Categorie Soggetti
Clinical Immunolgy & Infectious Disease",Microbiology
Journal title
JOURNAL OF CLINICAL MICROBIOLOGY
ISSN journal
00951137 → ACNP
Volume
39
Issue
10
Year of publication
2001
Pages
3750 - 3752
Database
ISI
SICI code
0095-1137(200110)39:10<3750:ROPIBS>2.0.ZU;2-N
Abstract
The effectiveness of PCR inhibitor removal by silica membranes in combinati on with the Amplicor Mycobacterium tuberculosis kit was analyzed for 655 re spiratory and nonrespiratory specimens. The overall inhibition rate was red uced from 12.5%, when applying the Amplicor kit alone, to 1.1% with the add ition of silica membrane DNA purification.